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Levi Lab @ USDA-ARS

Amnon Levi is a geneticist and project leader at the USDA-ARS Vegetable research laboratory in Charleston, SC, the CucCAP Watermelon team leader, and a CucCAP Bioinformatics team member. Sandra Branham is a postdoctoral research scientist in the lab.

CucCAP scientists study effect of genome doubling on gene expression in tetraploid watermelon

August 3, 2021August 19, 2021 cuccap CucCAP Watermelon Team, Featured Articles

Umesh Reddy and team at WVSU and Amnon Levi and team at USDA, ARS used advance genome sequencing technology to study the effect of genome doubling on gene expression in tetraploid watermelon versus their counterpart diploid watermelon parental plants.

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Watermelon researchers identify metabolic pathways and genome sequence

May 28, 2021May 28, 2021 cuccap CucCAP Watermelon Team

CucCAP researcher Amnon Levi collaborated with other scientists at the USDA ARS to develop a pioneering concept of using big data and computational biology to identify and catalog all of the phytochemicals that exist in edible fruit.

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XIIth Eucarpia Meeting on Cucurbit Genetics and Breeding

CucCAP scientist, Rebecca Grumet, will present,  “Application of genomic tools for mapping and analysis of valuable traits in cucurbit” at the XIIth Eucarpia Meeting on Cucurbit Genetics and Breeding.

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Meet the CucCAP Watermelon Team

February 10, 2021April 21, 2021 cuccap CucCAP Watermelon Team

The CucCAP Watermelon team is led by USDA-ARS Research Geneticist Amnon Levi. Co-investigators include Sandra Branham, Cecelia McGregor, Kai-Shu Ling, Shaker Kousik, Umesh Reddy, and Pat Wechter.

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Call for Papers| 2020 Cucurbit Genetics Cooperative report

January 16, 2021April 30, 2021 cuccap Cucurbit Genetics, Genomics, & Pathology

The cucurbit research community is encouraged to continue to share research, manuscripts, reports, descriptions of new techniques, interesting observations from their regions, or any information that could be of use or interest to the cucurbit genetics and related research communities.

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Cucurbit Genetics Cooperative Report 42

September 18, 2020November 16, 2020 cuccap CucCAP Project

CucCAP scientists and other researchers authored reports in the 2019 Cucurbit Genetics Cooperative (CGC) report which was published in September 2020.

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CucCAP researchers identify watermelon root traits to improve tolerance to stress and diseases

September 7, 2020March 22, 2022 cuccap CucCAP Watermelon Team, Featured Articles

In a recent study, CucCAP researchers used a digital imaging technology to evaluate the root systems in 335 watermelon accessions.

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CucCAP scientists uncover QTL associated with sulfur tolerance in melon, facilitating disease control options

August 7, 2020March 22, 2022 cuccap CucCAP Melon Team, Featured Articles

CucCAP researchers identified a major QTL associated with sulfur tolerance in a melon mapping population. Genetic markers were developed for the major QTL and can be used to incorporate sulfur tolerance in melon breeding programs.

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Featured Article

CucCAP scientists identify markers for powdery mildew resistance in watermelon

Powdery mildew outbreaks, caused by Podosphaera xanthii, cause reduced yields and fruit quality in watermelon. CucCAP scientists at Clemson University and the USDA Vegetable Laboratory Charleston SC employed a bulked segregant analysis approach using data from 1147 accessions from the USDA Citrullus germplasm collection to perform an extreme-phenotype genome-wide association study (XP-GWAS) of tolerance to P. xanthii race 2W. Kompetitive Allele-Specific PCR (KASP) markers were designed for significant SNPs associated with reduced disease. The best marker in each region explained 21-31% of the variation in powdery mildew tolerance.

For further reading:

Branham et al., 2025. Extreme-phenotype genome-wide association study (XP-GWAS) of powdery mildew race 2 W tolerance in the USDA Citrullus germplasm collection. Scientific Reports. DOI10.1038/s41598-025-89445-8

 

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USDA National Institute of Food and Agriculture U.S. DEPARTMENT OF AGRICULTURE

The Cucurbit Coordinated Agricultural Project (CucCAP) is a USDA-National Institute of Food and Agriculture Specialty Crop Research Initiative grant under award number
2020-51181-32139.

Cooperating Institutions:

Boyce Thompson Institute; Cornell University; Michigan State University; North Carolina State University; Clemson University; University of Puerto Rico; University of Wisconsin; University of Florida; University of Georgia; West Virginia State University; U.S. Department of Agriculture, Agricultural Research Service

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