CucCAP researchers develop new methods for detection and analysis of downy mildew pathogen on cucumber

Researchers in Dr. Mary Hausbeck’s group at MSU have developed a highly sensitive DNA-based protocol to genetically distinguish between spores of cucumber downy mildew and hop downy mildew from spore trap samples. The enhanced sensitivity enables detection 5-10 days before symptoms appear in cucumber fields, providing a broader window for initiation of chemical control.

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CucCAP scientists translate genomic studies into novel detection method for precision management of downy mildew outbreaks

Downy mildew caused by Pseudoperonospora cubensis is the most destructive foliar disease affecting cucurbit crops.  Genomic studies by Dr. Lina Quesada-Ocampo and colleagues at North Carolina State University showed that different isolates preferentially infect different cucurbit crops.  From this information, a multiplex PCR-based assay was combined with spore trapping to identify which crops are most at risk.  These results can facilitate timely and crop-specific fungicide application prior to appearance of symptoms in sentinel plots.

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