CucCAP scientists identified candidate gene contributing to resistance to downy mildew in cucumber

Downy mildew (DM) is an important disease of cucumber and other cucurbits. Many quantitative trait loci (QTL) have been identified from different resistant resources but very few have been fine mapped or cloned. CucCAP researchers in USDA-ARS and the University of Wisconsin Madison reported identification and functional characterization of the candidate gene for the major-effect QTL, dm5.3, for DM resistance from the plant introduction line PI 197088. This gene, which can enhance plant disease signaling, is currently being used to breed for disease resistance in cucumber.

Continue reading


CucCAP researchers develop high-throughput method for quantification of pathogen growth

Researchers at Michigan State University used a fluorescent isolate of the broad host range pathogen, Phytophthora capsici, to develop a high-throughput, microtiter plate assay for replicated quantification of  pathogen growth on plant tissue in real time.  This method, which can detect pathogen growth prior to development of symptoms, can assist with screening for disease resistance, mapping of resistance loci, testing efficacy of control measures, or elucidation of fundamental host-pathogen interactions.

Continue reading