CucCAP researchers develop new methods for detection and analysis of downy mildew pathogen on cucumber

Researchers in Dr. Mary Hausbeck’s group at MSU have developed a highly sensitive DNA-based protocol to genetically distinguish between spores of cucumber downy mildew and hop downy mildew from spore trap samples. The enhanced sensitivity enables detection 5-10 days before symptoms appear in cucumber fields, providing a broader window for initiation of chemical control.

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CucCAP scientists translate genomic studies into novel detection method for precision management of downy mildew outbreaks

Downy mildew caused by Pseudoperonospora cubensis is the most destructive foliar disease affecting cucurbit crops.  Genomic studies by Dr. Lina Quesada-Ocampo and colleagues at North Carolina State University showed that different isolates preferentially infect different cucurbit crops.  From this information, a multiplex PCR-based assay was combined with spore trapping to identify which crops are most at risk.  These results can facilitate timely and crop-specific fungicide application prior to appearance of symptoms in sentinel plots.

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Occurrence of Foliar Pathogens of Watermelon on Commercial Farms in South Carolina Estimated with Stratified Cluster Sampling | APS Journals

A survey of foliar pathogens of watermelon based on two-stage cluster sampling was conducted on commercial farms in South Carolina in spring 2015, spring and fall 2016, and fall 2017. In total, 60 fields from 27 different growers in seven counties representing the main watermelon-producing areas in the state were sampled, using a stratified two-stage […]

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August 3 Vegetable Update | NC State

Vegetable Production Updates Downy mildew was reported on cucumbers, cantaloupe, and butternut squash in sentinel plots at the Mountain Research Station in Haywood County. Downy mildew was also reported on cucumbers in sentinel plots in Henderson County. Southern blight was reported on cucurbits, peppers, and tomatoes in Western North Carolina. In most cases, southern blight […]

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